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. Author manuscript; available in PMC: 2024 Aug 21.
Published in final edited form as: Appl Microbiol Biotechnol. 2012 Jan 22;96(3):711–727. doi: 10.1007/s00253-012-3869-7

Figure 2. Plasmid map used for the construction of E. coli expression vector and primer sequences used for PCR-amplification of DGAT2 insert.

Figure 2

(A) Plasmid pMBP-hTTP [37] was used to express fulllengthDGAT2 in E. coli. Tung DGAT2 DNA was subcloned as described in the Materials and Methods section. (B) Primers for construction of E. coli and yeast expression plasmids. The sequences for restriction enzyme digestion sites are underlined. DGAT2 forward primer has a sequence coding for PreScission protease digestion site (5-CTGTTTCAGGGTCCG-3). DGAT2 reverse primer has a sequence coding for 6 histidine residues (5-ATGATGATGATGATGATG-3).