REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-Puromycin	Kerafast	EQ0001; RRID: AB_2620162
Rabbit anti-RpS12	Proteintech	16490-1-AP; RRID: AB_2146233
Rabbit anti-RpL24	Proteintech	17082-1-AP; RRID: AB_2181728
Rabbit anti-Trim46	Van Beuningen et al.14	N/A
Mouse anti-V5	Thermo Fisher Scientific	R960-25; RRID: AB_2556564
Rat anti-HA	Roche	11867423001; RRID: AB_390918
Mouse anti-GFP	Thermo Fisher Scientific	A-11120; RRID: AB_221568
Rabbit anti-GFP	Abcam	ab289; RRID: AB_303395
Streptavidin, Alexa-Fluor555 conjugate	Thermo Fisher Scientific	S21381; RRID: AB_2307336
Streptavidin, Alexa-Fluor568 conjugate	Thermo Fisher Scientific	S11226; RRID: AB_2315774
Goat-anti-Mouse Alexa Fluor 488	Thermo Fisher Scientific	A11029; RRID: AB_2534088
Goat-anti-Mouse Alexa Fluor 568	Thermo Fisher Scientific	A11031; RRID: AB_144696
Goat-anti-Mouse Alexa Fluor 405	Thermo Fisher Scientific	A31553; RRID: AB_221604
Goat-anti-Rabbit Alexa Fluor 647	Thermo Fisher Scientific	A21245; RRID: AB_2535813
Goat-anti-Rat Alexa Fluor 488	Thermo Fisher Scientific	A11006; RRID: AB_2534074
Goat-anti-Rabbit IRDye 800CW	Li-Cor	926-32211; RRID: AB_621848
Atto488 Fluotag-X4 GFP nanobody	NanoTag Biotechnologies	N0304-At488-L; RRID: AB_3075905
Goat-anti-Rabbit CF594	Sigma-Aldrich	SAB4600110
Goat-anti-Mouse STAR635P	Abberior	ST635P-1001; RRID: AB_2893232
Goat-anti-Mouse CF680	Biotium	20065; RRID: AB_10557108
Goat-anti-Rabbit Atto 647N	Sigma-Aldrich	40839; RRID: AB_1137669
Chemicals, peptides, and recombinant proteins
Puromycin dihydrochloride	Sigma-Aldrich	P8833
Anisomycin	Sigma-Aldrich	9789
recombinant human Neurotrophin-3	Alomone Labs	N-260
recombinant human BDNF protein	Alomone Labs	B-250
recombinant rat beta-NGF protein	R&D Systems	556-NG
NeutrAvidin	Thermo Fisher Scientific	31000
Hemin (Heme)	Sigma-Aldrich	51280
Biotin-phenol	Iris Biotech	LS.3500
Hydrogen Peroxide	Sigma-Aldrich	H1009
Lipofectamine2000	Thermo Fisher Scientific	11668019
Polyethylenimine (PEI MAX)	Polysciences	24765
Fluoromount-G Mounting Medium	Thermo Fisher Scientific	00-4958-02
Dynabeads M-280 Streptavidin	Thermo Fisher Scientific	11205D
Pierce Streptavidin Magnetic beads	Thermo Fisher Scientific	88816
RNase A	Thermo Fisher Scientific	EN0531
RNase T1	Thermo Fisher Scientific	EN0541
SUPERase-in RNase Inhibitor	Thermo Fisher Scientific	AM2694
Trolox	Sigma-Aldrich	238831
Sodium L-Ascorbate	Sigma-Aldrich	A4034
Sodium Azide	Sigma-Aldrich	S2002
Cysteamine (MEA)	Sigma-Aldrich	30070
Glucose-oxidase	Sigma-Aldrich	G2133
Catalase	Sigma-Aldrich	C40
Acryloyl-X-SE	Thermo Fisher Scientific	A20770
Acrylamide 40%	Sigma-Aldrich	A4058
N,N′-Methylenebisacrylamide	Sigma-Aldrich	M1533
Sodium acrylate	Sigma-Aldrich	408220
TEMED	Bio-Rad	1610800
APS	Sigma-Aldrich	A3678
anhydrous-DMSO	Thermo Fisher Scientific	D12345
Guanidine HCl	Sigma-Aldrich	G3272
Triton-X-100	Sigma-Aldrich	93433
Proteinase K	Thermo Fisher Scientific	EO0492
0.1% (w/v) poly-L-lysine	Sigma-Aldrich	P8920
Deposited data
Proteomics data P180 pulldown	This study	PXD050948
RNA-sequencing data P180	This study	GSE262262
Experimental models: Cell lines
HEK-293T	ATCC	CRL-3216
Experimental models: Organisms/strains
Rat (Wistar)	Janvier	N/A
Recombinant DNA
pSuper	Brummelkamp et al.74	N/A
pGW1-mCherry	Kapitein et al.75	N/A
pGW1-BFP	Kapitein et al.75	N/A
RTN4A-GFP	Kind gift from dr. Voeltz (Howard Hughes Medical Institute & Department of Molecular, Cellular and Developmental Biology, University of Colorado)	Addgene #61807
pEGFP(A206K)-N1	Kind gift from dr. Lippincott-Schwartz- (Howard Hughes Medical Institute, Janelia Research Campus)	N/A
pEGFP(A206K)-C1	Kind gift from dr. Lippincott-Schwartz (Howard Hughes Medical Institute, Janelia Research Campus)	N/A
GFP-Sec61β	Kind gift from dr. Rapaport (Department of Cell Biology, Harvard Medical School)	Addgene #15108
DP1-GFP	Farías et al.12	N/A
RpL10A-tagRFP	Kind gift from dr. Singer (Department of Cell Biology, Albert Einstein College of Medicine)	Addgene #74172
TOM20-V5-FKBP-AP	Kind gift from dr. Ting (Department of Genetics& Biology, Stanford University)	Addgene #120914
mCherrymyr5’/3’-Calreticulin	Kind gift from dr. Twiss (Department Biological Sciences, University of South Carolina)	N/A
Lifeact-mCherry	Kind gift from dr. Sato (Graduate School of Arts and Sciences, University of Tokyo)	Addgene #67302
HA-KifC1-MD-Strep	Farías et al.12	N/A
GFP-SBP-RTN4A	Farías et al.12	N/A
P180-ΔCoiled-coil-GFP	Farías et al.12	N/A
P180-ΔCoiled-coil-GFPAviTag	This study	N/A
P180-Δrepeats-GFP	Farías et al.12	N/A
P180-Δrepeats-GFPAviTag	This study	N/A
P180-Coiled-coil-GFPAviTag	This study	N/A
P180-Repeats-GFPAviTag	This study	N/A
SplitAP-V5-C1	Özkan et al.19	N/A
3xHA-split-EX-N1	Özkan et al.19	N/A
GFP-LRRC59	This study	N/A
RPN1-GFP	This study	N/A
KTN1-mNG	This study	N/A
P180-RCC-GFP	This study	N/A
P180-RCC-GFPAviTag	This study	N/A
Split-AP-V5-Sec61β	This study	N/A
RpL10A-3xHA-Split-EX	This study	N/A
P180-V5-Split-AP	This study	N/A
Split-AP-V5-RTN4	This study	N/A
pSuper-rat RTN4: shRNA targeting sequence: gtccagatttctctaatta	Farías et al.12	N/A
pSuper-rat DP1: shRNA targeting sequence: gacatataaagttccagaa	Farías et al.12	N/A
pSuper-rat P180-1: shRNA targeting sequence: tcagtgcaattgtctgtat	Özkan et al.19	N/A
pSuper-rat P180-2: shRNA targeting sequence: taaaccaaccaacacagcg	Özkan et al.19	N/A
Software and algorithms
Fiji/ImageJ2	v2.14/1.54f	https://fiji.sc; RRID: SCR_002285
R with R Studio	v4.2.2	https://www.r-project.org
GraphPad Prism	GraphPad; v10	https://www.graphpad.com