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. 2004 Jul 6;381(Pt 2):463–469. doi: 10.1042/BJ20031083

Figure 6. Annexin II association with caveolin is stearate/acylation-dependent.

Figure 6

Cells in 10 cm plates were serum-starved for 48 h and treated with or without 50 μM stearate for 6 h, and DRM and DSM were prepared. Caveolin and caveolin-associated proteins were immunoprecipitated with caveolin IgG (5 μg/sample) as indicated (immunoprecipitation antibody) and resolved by SDS/PAGE. The proteins were transferred on to PVDF and immunoblotted for annexin II and caveolin. In lanes 1, 3, 5 and 7, non-immune mouse IgG was used for immunoprecipitation as a negative control. Arrows on the right indicate the antibody heavy and light chains, whereas the arrows on the left indicate annexin II (upper) and caveolin (lower). The experiment shown is representative of three separate experiments.