Table 1. Incorporation of 32P into specific in vitro phosphorylation sites in DCX by cdk5.
Cerenkov radiation (c.p.m.) | % c.p.m. | Summed fractions | Phosphorylation site | Sequence (phospho-site in bold) | HPLC fraction (Figure 2a) |
---|---|---|---|---|---|
4360 | 43 | 17–23(1/2) | Ser-339, Ser-332 (minor) | …KSKQSPISTPTSPGSL… | iv, v |
2399 | 24 | 23(1/2)–28 | Ser-28 | …NGLPSPTHS… | vi, vii |
1289 | 13 | 4–5 | Ser-287, Thr-289 | …GPKASPTPQKT… | i |
1269 | 13 | 8–10 | Ser-297 | …TSAKSPGPM… | ii |
783 | 8 | 12–14 | Thr-326 | …SQLSTPKSK… | iii |