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Double-immunofluorescence labelling with anti-KGA (green) and endocrine cell markers: insulin, glucagon or somatostatin (red). In confocal overlay images, yellow represents co-localization of the two antigens. (A3) KGA and insulin, (B3) KGA and glucagon and (C3) KGA and somatostatin. KGA was present in α- and δ-cells (B3 and C3 respectively) but was not detected in insulin-containing cells (A3). Scale bar, 57 μm.
