Figure 4. LPS induces stabilization of IL-1F7b mRNA in transfected RAW264.7/IL-1F7b cells.

Subclones of RAW264.7 cells stably transfected with IL-1F7b (A) or a control gene (S3rev) (B) were stimulated with LPS (10 ng/ml). Total RNA was isolated after the indicated time and reverse-transcribed as described. Semi-quantitative PCR was performed and the product was applied to a 1% agarose gel. Results from a single experiment out of at least three representative experiments are shown.