Table 3. Primary antibodies used in the IHC panel for primary ureteral malignant leiomyosarcoma in a mixed-breed dog.

Antigen	Clone	Dilution	Antigen retrieval	Source	Reactivity
Alpha-smooth muscle actin	1A4	1:1000	High pH link, DAKO	K800221, Agilent DAKO	Positive
Smooth muscle myosin heavy chain	SMMS-1	Ready-to-use	High pH link, DAKO	K800221, Agilent DAKO	Positive
Actin, muscle specific	HHF-35	Ready-to-use	High pH link, DAKO	K800221, Agilent DAKO	Positive
Desmin	D33	Ready-to-use	High pH link, DAKO	K800221, Agilent DAKO	Positive
Myogenin	F5D	Ready-to-use	High pH Link, DAKO	K800221, Agilent DAKO	Negative
Pancytokeratin	AE1AE3	Ready-to-use	High pH link, DAKO	K800221, Agilent DAKO	Negative
Uroplakin III	AU1 (UPIII)	1:20	High pH link, DAKO	Fitzgerald	Negative
GATA binding protein 3 (GATA3)	L50-823	1:50	High pH link, DAKO	Biocare Medical	Negative
SOX10	BC34	Ready-to-Use	High pH link, DAKO	Cell Marque	Negative
GFAP	Polyclonal	Ready-to-use	High pH link, DAKO	K800221, Agilent DAKO	Negative

GFAP, glial fibrillary acidic protein.