Figure 1. Intracellular transport of human LAMAN.

(A) COS-7 cells were transfected with pcDNA constructs containing wild-type human LAMAN cDNA using LIPOFECTAMINE™ 2000. The cells were pulsed 48 h later with [³⁵S]methionine/cysteine for 30 min and the cells were subsequently harvested or chased for 5 h. Human LAMAN was precipitated from the cell lysates or the media with a polyclonal antiserum [7]. The resulting immunoprecipitates were treated with endo H or PNGase F, separated by SDS/PAGE and analysed by autoradiography. The positions of the respective LAMAN peptide fragments are indicated on the left. (B) A model of the intracellular transport of human LAMAN in COS cells, based on the results shown in (A).