Figure 2. Secretion kinetics of human LAMAN.

COS-7 cells were transfected with pcDNA constructs containing a human LAMAN cDNA construct as described in Figure 1. Subsequent to the pulse period of 30 min with [³⁵S]methionine/cysteine, the cells were chased for the indicated times. Human LAMAN was immunoprecipitated from the medium and analysed by autoradiography. The bands were scanned and the intensity of the respective bands was correlated with that of the band representing the 24 h chase.