Table 1. Primers used for the construction of mutants.

The substituted nucleotides are indicated in boldface and dashes indicate the positions at which deletions were introduced to mimic the effect of the splice site mutations [2].

Primer	Direction	Sequence
H72L	Forward	5′-GCCTCACACACTTGATGACGTG-3′
	Reverse	5′-CACGTCATCAAGTGTGTGAGGC-3′
D196E	Forward	5′-GTGGCCTGGCACATTGAGCCCTTCGGCCAC-3′
	Reverse	5′-GTGGCCGAAGGGCTCAATGTGCCAGGCCAC-3′
D196N	Forward	5′-GTGGCCTGGCACATTAACCCCTTCGGCCAC-3′
	Reverse	5′-GTGGCCGAAGGGGTTAATGTGCCAGGCCAC-3′
R220H	Forward	5′-GACGGCTTCTTCTTTGGGCACCTTGATTATCAAG-3′
	Reverse	5′-CTTGATAATCAAGGTGCCCAAAGAAGAAGCCGTC-3′
Del(256–260) (IVS5−1G-C)	Forward	5′-GACCTCTTCAC_TGGTTACAACCCGCC-3′
	Reverse	5′-GGCGGGTTGTAACCA_GTGAAGAGGTC-3′
F320L	Forward	5′-CATGGGCTCGGACCTCCAATATGAGAATGC-3′
	Reverse	5′-GCATTCTCATATTGGAGGTCCGAGCCCATG-3′
Del(339–342) (IVS7+2T-G)	Forward	5′-CTCATCCGGCT_GCAGGCAAAAGGAAG-3′
	Reverse	5′-CTTCCTTTTGCCTGC_AGCCGGATGAG-3′
T355P	Forward	5′-GTTCTCTACTCCCCCCCCGCTTGTTAC-3′
	Reverse	5′-GTAACAAGCGGGGGGGGAGTAGAGAAC-3′
P356R	Forward	5′-CTCTACTCCACCCGCGCTTGTTACCTC-3′
	Reverse	5′-GAGGTAACAAGCGCGGGTGGAGTAGAG-3′
E402K	Forward	5′-CCCTCAAACGCTACAAGCGCCTCAGC-3′
	Reverse	5′-GCTGAGGCGCTTGTAGCGTTTGAGGG-3′
W714R	Forward	5′-CCTGGAGCTAGAGCGGTCGGTGGGG-3′
	Reverse	5′-CCCCACCGACCGCTCTAGCTCCAGG-3′
R750W	Forward	5′-GACAGCAATGGCTGGGAGATCCTGGAG-3′
	Reverse	5′-CTCCAGGATCTCCCAGCCATTGCTGTC-3′
L809P	Forward	5′-GAGATGGCTCGCCGGAGCTCATGGTGC-3′
	Reverse	5′-GCACCATGAGCTCCGGCGAGCCATCTC-3′