Figure 5. 3D structure of one enzyme subunit of the testis PFK-2/FBPase-2 isoenzyme.
The co-ordinates were retrieved from the PDB database (accession code 1BIF) containing ATPγS in the PFK-2 domain. The position of Fru-6-P was modelled as described in [44]. In the upper right hand PFK-2 domain, ATP is on the left and Fru-6-P is on the right. In the lower left hand FBPase-2 domain, two inorganic phosphates indicate the position of the Fru-2,6-P2-binding site. The PFK-2 domain is composed of a β-sheet surrounded by α-helices. Two subdomains, composed of α-helices (above) form a flexible cover and are involved in Fru-6-P binding and catalysis (induced fit, see text).