Figure 5 .

Coefficient of variation analysis of Raman spectra during myocardial differentiation of six hiPSC-lines before induction of differentiation (Day-0). (A) Spectra of coefficient of variation (CV) of six hiPSC-lines. Color from yellow to green correspond to value from 0 to 1.0. (B) Time developments of histogram of CV at the peaks of 755 cm^–1 peaks (left), 707 cm^–1 peak (middle) and 940 cm^–1 peak (right). Scale bar indicates 10% frequency. All six cell-lines were used for analysis. (C) A heatmap of the correlations of time development of CV Correlation of CV time development between 940 cm^–1 (upper) or 1340 cm^–1 (lower) was calculated against other peaks and visualized. Color from blue to red correspond to value from –1.0 to 1.0. (D) Principal component analysis of mean intensity of Raman spectra in a dish (upper) and that of CV (lower). Each plot indicates each dish. Color is the same to Figure 4B.