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. 2004 Jul 27;381(Pt 3):645–653. doi: 10.1042/BJ20040280

Figure 2. Production of the SsoCdc6-2 truncated forms.

Figure 2

(A) Schematic representation of the polypeptide chain of SsoCdc6-2 and its C- (SsoCdc6-2 ΔC) and N-terminally (SsoCdc6-2 ΔN) deleted forms. W-A and W-B stand for Walker A and Walker B motifs respectively. S1 and S2 stand for Sensor 1 and Sensor 2 respectively. (B) SsoCdc6-2 and its truncated forms were produced as recombinant proteins and purified as described in the Experimental section. Samples of the homogeneous proteins (5 μg) were subjected to SDS/15% PAGE. Protein bands were detected by Coomassie-Blue-staining of the gel.