Figure 4. ATPase activity of the SsoCdc6-2 and its truncated forms.

ATPase activity assays were carried out at 60 °C for 30 min using increasing amounts of SsoCdc6-2, ΔC or ΔN in the presence of radiolabelled [γ-³²P]ATP and 5 mM ATP as described in the Experimental section. The orthophosphate (P_i) released during the hydrolysis reaction was plotted against the amount of protein used. Results are mean values of at least three independent experiments.