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The ability of SsoCdc6-2 to bind a 32P-labelled 51-mer synthetic oligonucleotide in a ds form was analysed by gel-shift assays in the presence of increasing concentrations of ATP. Detection and quantification of the radioactive bands was carried out using a PhosphorImager. A plot of the shifted DNA against the amount of protein used is shown. Results are mean values of at least three independent experiments.
