Figure 7. Partial trypsin proteolysis of SsoCdc6-2.

Aliquots of homogeneous SsoCdc6-2 (3 μg) were subjected to partial trypsin digestion as described in the Experimental section. Reactions (20 μl volume) were carried out in the absence (lanes 2–5) or in the presence (lanes 8–11) of 5 mM ATP with increasing amounts of protease (0–100 ng, lanes 2–5 and 8–11). Samples were analysed by SDS/12.5% PAGE and Coomassie-Blue-staining.