Figure 9. MEKK1 is glutathionylated at Cys¹²³⁸ in menadione-treated cells.

MEKK1 was purified from untreated cells or from cells treated with 250 μM menadione, digested with trypsin and proteinase Asp-N and analysed by MS. (A) Predicted production (MS/MS) values for unmodified and glutathionylated Cys¹²³⁸-containing peptide. Representative MS/MS spectra for unmodified peptide (B) and glutathionylated peptide (C) are shown, with indicators identifying the predicted peaks.