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. 2004 Jul 27;381(Pt 3):725–733. doi: 10.1042/BJ20040228

Figure 3. Sensorgram depicting the binding of triclosan to ENR in the absence (A) and presence (B) of NAD+.

Figure 3

(A) Various concentrations of triclosan (0.4, 0.8, 1, 1.5, 3 and 4 μM) were passed over the immobilized PfENR. The surfaces were regenerated by a 10-s pulse of 10 mM glycine/HCl (pH 4.2) flowing at 50 μl/min. The fit of the sensorgram yielded association and dissociation values of 741 M−1·s−1 and 6.84×10−3 s−1 respectively, and an Rmax of 150. (B) NAD+ was passed over immobilized PfENR for 1 h followed by varying concentrations of triclosan (0.4, 0.8, 1, 1.5 and 3 μM). The fit of the curve yielded forward and reverse rate constants of 1.3×104 M−1·s−1and 4.2×10−4 s−1 respectively, and an Rmax of 175.