Figure 4. Sensorgram depicting the binding of NAD⁺ to ENR in the presence of triclosan.

Triclosan (50 μM) was passed over immobilized PfENR for 1 h followed by varying concentrations of NAD⁺ (5, 10, 15, 20 and 25 μM). The surfaces were regenerated by a 10-s pulse of 10 mM glycine/HCl (pH 4.2) flowing at 50 μl/min. The fit of the sensorgram yielded association and dissociation values of 1.9×10³ M⁻¹·s⁻¹ and 0.029 s⁻¹ respectively, and an R_max of 60.