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. 2004 Jul 27;381(Pt 3):831–840. doi: 10.1042/BJ20031930

Figure 2. Soluble form of lobster HMGR has a molecular mass of approx. 500 kDa.

Figure 2

The 100000 g supernatant was analysed by gel filtration (Superdex 200 HR 10/30 column, 1 ml fraction) and HMGR activity was detected in several fractions, with the highest activity eluting in fraction 11 (estimated molecular mass approx. 500 kDa) and a minor peak in fraction 16 (estimated molecular mass approx. 120 kDa). Inset: elution volumes of the molecular standards and the peaks of HMGR activity (arrows). This Figure is representative of the results obtained in five experiments.