Skip to main content
. 2004 Jul 27;381(Pt 3):831–840. doi: 10.1042/BJ20031930

Figure 6. Recombinant HMGR1 (rec-HMGR1) is expressed in Sf9 cells.

Figure 6

Cells were infected with a recombinant baculovirus containing HMGR1 cDNA. The rec-HMGR1 was purified from the cell homogenates by affinity chromatography using the His tag. (A) Aliquots of the infected Sf9 cell homogenates, the 100000 g supernatant and the His-tag purified protein were analysed by SDS/PAGE (lanes 1–3 respectively). (B) Western blot of a similar gel using Psm-HMGR antiserum showed one immunoreactive protein band of 69 kDa. Molecular masses of the protein standards are shown on the right side of the SDS/polyacrylamide gel; the calculated masses of the immunoreactive bands are given for the Western blots. The Figure is representative of the three analyses of these fractions.