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. 2024 Jul 19;32(3):200850. doi: 10.1016/j.omton.2024.200850

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© 2024 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Screening of scFv immune library for TCRA6 binders by FACS

Sorting rounds 1–3 of the chicken-derived yeast surface-displayed scFv library are depicted in green with respective TCRA6 concentrations and percentages of cells in sorting gates. Negative controls without antigen incubation are shown in gray. Surface presentation was detected by anti-c-myc antibody FITC conjugate, and antigen binding was analyzed using DyLight650-labeled TCRA6. Density plots were created by FlowJo v10 Software.