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. 2004 Jul 27;381(Pt 3):853–859. doi: 10.1042/BJ20040443

Figure 4. Five alanine mutations affecting the production and/or stability of PSS 1.

Figure 4

CHO-K1 cells were transiently transfected with 6 μg of each of the control empty vector plasmid (vector) or plasmids that encoded wild-type (wt) or Y111A, D166A, R184A, R323A and E364A mutant PSS 1. (A) Cell lysates of the transfectants were assayed for serine base-exchange activity. Three independent experiments gave similar results; the values shown are for one of the three independent experiments, and are the averages of duplicate determinations with a variation of <12% between duplicates. (B) Cell lysates (10 μg of protein) of the transfectants were separated by SDS/PAGE, and then analysed by Western blotting with an anti-PSS 1 N-terminal peptide antibody.