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. 2004 Jul 27;381(Pt 3):895–904. doi: 10.1042/BJ20040435

Figure 1. Interaction of collagen C-propeptides with the PDZ domain of HtrA1.

Figure 1

(A) Interaction of Type I, II and III collagen with HtrA1. One clone encoding almost the same size of Type I, II or III collagen α1 molecule (each encoding C-terminal 365, 359 or 345 amino acids respectively) was selected from the pACT2 collagen clones isolated by yeast two-hybrid screening. They were analysed for interaction with HtrA1 by the β-galactosidase liquid assay of yeast two-hybrid using pGBT9-ΔSS-HtrA1 (closed bar) or the empty pGBT9 vector (open bar) as bait. (B) Specific interaction of Col3a1 with the PDZ domain of HtrA1. Various constructs of HtrA1 in pGBT9 as indicated were analysed for interaction with the Col3a1 clone in pACT2, as described in (A). (C) Interaction of the C-terminal end of Col3a1-C-Pro with HtrA1. The various constructs of Col3a1 in pACT2 as indicated were analysed for interaction with HtrA1 using pGBT9-ΔSS-HtrA1 as bait, as described in (A). SS, signal sequence; IB, IGF-binding-protein-like domain; KI, Kazal-type protease inhibitor domain; SP, serine protease domain; N, non-helical region of mature collagen.