Table 1. Genes isolated from yeast two-hybrid screening.
The day-17 mouse embryo cDNA library and the adult mouse brain cDNA library, 3.5×106 clones each, were screened for PDZ-binding proteins by yeast two-hybrid system as described in the Experimental section. The identity of WAC (WW domain-containing adapter with a coiled-coil region) has been obtained only recently and therefore this clone was not analysed in this study.
| Gene | Number of isolated clones | C-terminal sequence | Accession number |
|---|---|---|---|
| Col3a1 | 29* | -GPVCFL-COOH | AK019448 |
| Col1a1 | 5* | -GPACFV-COOH | BC003198 |
| Col2a1 | 2* | -GPVCFL-COOH | NM_031163 |
| GM130 | 15*† | -VKIMVV-COOH | BC011407 |
| Cdyl | 6† | -RKIDEF-COOH | NM_009881 |
| LRP9 | 3* | -DEPLLA-COOH | AB042200 |
| CoxVa | 3* | -LGLDKV-COOH | NM_007747 |
| CAST | 3† | -EEGIWA-COOH | AK122265 |
| mKIAA0769 | 3† | -VEITLV-COOH | AB018312 |
| PAR6B | 1* | -GTIITL-COOH | NM_021409 |
| WAC | 3† | -QNSFMF-COOH | AF320996 |
* Clones isolated from the embryo library.
† Clones isolated from the adult brain library.