. 2004 Jul 27;381(Pt 3):905–909. doi: 10.1042/BJ20040433

Table 1. Substrate K_m and active analogue K_i studies on native variants and mutants of PvHT.

Values (means±S.E.M.) are expressed in mM and are based on results of at least three independent replicates. K_i values of active analogues are based on inhibition of D-glucose uptake.

Substrate or competitor	PfHT	PvHT_ind	PvHT_ind Q167N	PvHT_ind chimaera	PvHT_sal
D-glucose	1.0±0.2 (K_m)^*	0.77±0.18 (K_m)^*	0.60±0.12 (K_m)	0.65±0.24 (K_m)	0.52±0.13 (K_m)
D-fructose	11.5±1.6 (K_m)^*	19.03±4.4 (K_i)	93.6±14.85 (K_i)	17.05±2.76 (K_i)	5.91±1.29 (K_i)
2,5-AHM	1.4±0.3 (K_i)^*	6.33±0.94 (K_i)	10.19±0.89 (K_i)	5.31±0.89 (K_i)	1.48±0.45 (K_i)
Compound 3361	0.053±0.02 (K_i)^*	0.12±0.01 (K_i)^*	0.52±0.09 (K_i)	0.18±0.05 (K_i)	0.13±0.01 (K_i)
2-DOG	1.3±0.3 (K_m)^*	2.1±0.4 (K_i)	1.81±0.2 (K_i)	1.45±0.12 (K_i)	1.24±0.24 (K_i)
6-DOG	2.2±0.9 (K_i)^*	11.74±3.04 (K_i)	8.03±1.3 (K_i)	10.52±2.43 (K_i)	3.61±0.83 (K_i)

* Previously reported data [8,9] (PfHT kinetic results have been reported previously, but are included for comparison).

Table 1. Substrate Km and active analogue Ki studies on native variants and mutants of PvHT.

Table 1. Substrate K_m and active analogue K_i studies on native variants and mutants of PvHT.