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. 2024 Jul 1;300(8):107516. doi: 10.1016/j.jbc.2024.107516

Figure 8.

Figure 8

Effect of ITGB3 inhibition by cyclo-RGDfK on HK-2 cells injury.A, verification of ITGB3 knockdown in HK-2 cells. RT-PCR analysis of ITGB3 (A) and Western blot analysis of integrinβ3 (B) in HK-2 cells treated with ITGB3-siRNA. C, Western blot analysis of integrinβ3, TSP-1, and KIM-1 in HK-2 cells treated with ITGB3-siRNA exposed to rTSP-1 (1 ug/ml) for 24 h. D, Western blot analysis of integrinβ3, TSP-1, and KIM-1 in HK-2 cells treated with ITGB3-siRNA exposed to BSA (20 mg/ml) for 24 h. E, Western blot analysis of integrinβ3, TSP-1, KIM-1, and α-SMA in HK-2 cells treated with cyclo-RGDfK (0.5 uM) and rTSP-1 (1 ug/ml) for 24 h. For statistical analysis, one-way ANOVA was used for A–C. ∗p < 0.05 compared with control cells. # p < 0.05 compared with cells exposed to BSA (20 mg/ml) or rTSP-1 (1 ug/ml). BSA, bovine serum albumin; Col-1, collagen I; rTSP-1, recombinant human TSP-1 protein; TSP-1, thrombospondin 1; ITGB3, integrin β3.