Figure 10. Cross-linking-induced insolubility of CD32A occurs in the absence of tyrosine phosphorylation.

CD32A was cross-linked on neutrophil membranes in the presence (+) or absence (−) of the kinase mixture (KM) and VO₄. Membranes were solubilized with 1% NP40 and after centrifugation (15000 g, 10 min), the supernatants (S) and pellets (P) were probed for an anti-pY or anti-CD32A antibody as described in the Materials and methods section.