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. 2004 Jul 27;381(Pt 3):919–928. doi: 10.1042/BJ20031808

Figure 3. Tyrosine phosphorylation of Syk and CD32A on neutrophil membranes after CD32A cross-linking.

Figure 3

Syk (A) and CD32A (B) were immunoprecipitated from neutrophil membranes after CD32A cross-linking, as described in the Materials and methods section. An aliquot of the 15000 g supernatant (S1) and an aliquot of the supernatant after immunoprecipitation (S2) were analysed alongside the immunoprecipitates (IP). AB, antibody alone. Samples were subsequently analysed by SDS/PAGE and immunoblotted with an anti-pY or CT10 antibody (anti-CD32A).