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. 2004 Jul 27;381(Pt 3):919–928. doi: 10.1042/BJ20031808

Figure 4. Cross-linking-induced insolubility of CD32A in non-ionic detergents on neutrophil membranes.

Figure 4

CD32A was cross-linked on neutrophil membranes as described in the Materials and methods section and membranes were solubilized in 1% NP40 and centrifuged at 15000 g for 10 min (A) or at 100000 g for 1 h (B). The supernatant (S) and pellet (P) were subsequently analysed by SDS/PAGE and immunoblotted with an anti-pY or CT10 antibody (anti-CD32A). The two major substrates whose tyrosine phosphorylation is enhanced are indicated with arrows.