Skip to main content
. 2004 Jul 27;381(Pt 3):919–928. doi: 10.1042/BJ20031808

Figure 5. Cross-linking of CD32A in isolated plasma membranes induces its recruitment to high-density DRMs.

Figure 5

CD32A was cross-linked on neutrophil membranes as described in the Materials and methods section. After NP40 solubilization, the membranes were subjected to ultracentrifugation on Optiprep gradients. Then, 13 fractions were collected and the proteins were precipitated and analysed by SDS/PAGE. (A) Membranes were immunoblotted with a CT10 (anti-CD32A) or anti-flotillin-1 antibody. (B) Membranes were immunoblotted with an anti-pY antibody.