Figure 6. Insolube CD32A translocates to high-density DRMs.

CD32A was cross-linked on neutrophil membranes as described in the Materials and methods section. After NP40 solubilization, membranes (600 μl) were ultracentrifuged for 1 h at 100000 g on 100 μl of a 48% Optiprep cushion. (A) The pellets were diluted to 40% Optiprep and subjected to Optiprep-gradient ultracentrifugation as in Figure 5. (B) The supernatants were immunoblotted with a CT10 antibody (anti-CD32A).