Figure 7. Inhibition by PP2 of the tyrosine phosphorylation response induced in plasma membranes after cross-linking of CD32A.

Plasma membranes were preincubated for 10 min in the absence or presence of 50 nM PP2. CD32A was then cross-linked as indicated in the Materials and methods section. Samples were probed with an anti-pY antibody. The two major substrates whose tyrosine phosphorylations are enhanced are indicated with arrows.