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. 2004 Jul 27;381(Pt 3):919–928. doi: 10.1042/BJ20031808

Figure 8. Solubilization profiles of CD32A and Src kinases on neutrophil membranes after cross-linking of CD32A.

Figure 8

CD32A was cross-linked on neutrophil membranes as described in the Materials and methods section. Membranes were then solubilized for 20 min in 1% NP40 on ice, 1% NP40 at 37 °C or 100 mM OGD on ice. After centrifugation (15000 g, 10 min), the supernatants (S) and pellets (P) were analysed by SDS/PAGE and probed for CD32A, Hck, Lyn or Fgr as described in the Materials and methods section. M, whole membranes before solubilization in detergents.