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. 2004 Jul 27;381(Pt 3):919–928. doi: 10.1042/BJ20031808

Figure 9. Effect of M-β-CD on tyrosine phosphorylation and translocation of CD32A to DRMs.

Figure 9

Plasma membranes were preincubated for 20 min in the absence or presence of 20 mM M-β-CD. CD32A was then cross-linked as indicated in the Materials and methods section. (A) Samples were probed with an anti-pY or anti-CD32A antibody. (B) After NP40 solubilization, the membranes were subjected to ultracentrifugation on Optiprep gradients as in Figure 5. Samples were immunoblotted (IB) with a CT10 (anti-CD32A) or anti-Lyn antibody. The two major substrates whose tyrosine phosphorylation is enhanced are indicated with arrows.