Table 1. Characterization of yeast nuclear fraction.
Nuclear membranes were isolated from YPH259 wild-type cells. Relative enrichment of marker proteins in the homogenate was set at 1. Antibodies against the following proteins were used: Kar2p, Nop1p, porin, Kex2p, carboxypeptidase Y and plasma membrane ATPase. Values represent averages of six independent preparations.
| Subcellular compartment | ||||||
|---|---|---|---|---|---|---|
| Nuclear membrane/ER | Mitochondria | Golgi | Vacuole | Plasma membrane | ||
| Marker protein | Kar2p | Nop1p | Porin | Kex2p | Carboxypeptidase Y | ATPase |
| Fold enrichment | 11 | 14 | 2 | 0.4 | 2 | 2 |