Fig. 1.

a–d Western blot and relative densitometry of TfR1 expression in brain cortical samples (a, c) and in microvessels (b, d) isolated from 5xFAD transgenic mice versus age-matched wild-type (WT) mice. Data are shown as the ratio between TfR1 and GAPDH as a reference loading control. Each bar represents the mean ± SEM of 7 animals per condition except for 5xFAD microvessels (n = 4); **p < 0.01 compared to WT mice, Student’s t-test. e Gene expression of transferrin receptor (Tfrc) in the brain cortical tissue of 5xFAD and wild-type (WT) mice. The data are presented as mean ± SEM of 7 animals per condition. The gene expression was normalized against the beta-actin (Actb) house-keeping gene