Fig. 4.

a–b Western blot and relative densitometry of TfR1 expression in hCMEC/D3 cells treated with 0.1 µM of Aβ1-40 and Aβ1-42 for 48 h compared to untreated cells (Ctrl). Data are shown as the ratio between TfR1 and GAPDH as reference loading control. Each bar represents the mean ± SEM of 4 samples. Student’s t-test. c Gene expression of transferrin receptor (TFRC) in hCMEC/D3 cells treated with 0.1 µM of Aβ1-40 and Aβ1-42 for 48 h compared to untreated cells (Ctrl). The data are presented as mean ± SEM of 6 samples per condition. The gene expression was normalized against the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) house-keeping gene. Student’s t-test