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Semi-quantitative RT–PCR was performed using a Drosophila Rapid-Scan™ panel, which includes cDNAs from 0–4, 4–8, 8–12 and 12–24 h old embryos, first, second and third instar larvae, pupae, male and female heads and male and female bodies. RT–PCR with the α4GT1-specific primers was performed with the 1000×dilution, whereas for rp49-specific primers the 100×dilution was used.
