Table 1. Various functional roles of CBP-interacting sites I and II of GRIP1 with GR, PR and TR in HeLa, HEK-293 and CV-1 cell lines.
Fold activation | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
CBP-interacting site status | GR+MMTV-LUC | PR+MMTV-LUC | TR+MMTV(TRE)-LUC | ||||||||
GRIP1 | I | II | HeLa | HEK-293 | CV-1 | HeLa | HEK-293 | CV-1 | HeLa* | HEK-293 | CV-1 |
Vector | − | − | 1±0.2 | 1±0.1 | 1±0.2 | 1±0.2 | 1±0.1 | 1±0.1 | 1±0.1 | 1±0.2 | 1±0.01 |
5–1462 | + | + | 10±1 | 1±0.1 | 32±1 | 5±0.6 | 19±2 | 12±0.2 | 17±2 | 5±0.3 | 5±0.7 |
5–1462 | − | + | 6±0.7 | 1±0.1 | 18±1 | 2±0.5 | 5±0.4 | 3±0.3 | 2±0.3 | 0.5±0.05 | 0.5±0.02 |
5–1462 | + | − | 13±1 | 1±0.1 | 24±0.2 | 5±0.5 | 29±0.2 | 13±1 | 44±8 | 6±0.6 | 3±0.7 |
563–1121 | + | + | 7±1 | 0.6±0.01 | 48±5 | 2±0.1 | 6±0.1 | 7±0.3 | 12±1 | 7±0.6 | 13±0.5 |
563–1121 | − | + | 6±0.1 | 1±0.01 | 34±1 | 1±0.5 | 6±0.4 | 5±0.2 | 1±0.1 | 0.3±0.01 | 0.7±0.09 |
563–1121 | + | − | 7±0.7 | 0.8±0.01 | 31±0.6 | 2±0.7 | 5±0.3 | 6±0.9 | 6±0.1 | 6±0.3 | 4±0.2 |
563–1094 | + | − | 9±0.4 | 1±0.05 | 36±1 | 2±0.1 | 7±0.4 | 8±1 | 6±0.2 | 6±0.2 | 4±0.4 |
563–1012 | − | − | 7±0.2 | 1±0.02 | 31±6 | 2±0.1 | 1±0.1 | 8±1 | 2±0.3 | 0.6±0.05 | 1±0.2 |
* Fold values are calculated based on the relative data shown in Figure 5(a).