Figure 1. Tryptic degradation of NDH-K of the Ndh complex in barley thylakoids.

Freshly prepared thylakoids were incubated with trypsin [360 units·(mg Chl)⁻¹] for 0 (T₀) or 20 min at 20 °C in the absence (1) or in the presence of Triton X-100 [8 mg·(mg of Chl)⁻¹] (2) or 140 mM NaCl (3). After SDS/PAGE (12% acrylamide) and blotting on to PVDF membranes, NDH-K polypeptide was revealed by a monospecific NDH-K antibody.