Figure 2. Effect of Triton X-100 on the tryptic degradation of NDH-A, ATPase α-subunit and D1 protein in barley thylakoids.

Freshly prepared thylakoids were incubated in the absence or presence of trypsin [360 units·(mg of Chl)⁻¹] and Triton X-100 [4 mg·(mg of Chl)⁻¹] for the indicated times at 20 °C. After SDS/PAGE (12% acrylamide) and blotting on to PVDF membranes, NDH-A, ATPase α-subunit, D1 and their tryptic derivatives were revealed by specific antibodies.