Figure 6. Proteinase V8 fragments of NDH-F containing the WSKDEILSNSWLYS sequence from intact and detergent-treated thylakoids.

Freshly prepared thylakoids were incubated with proteinase V8 [0.2 unit·(mg of Chl)⁻¹] for the indicated times at 30 °C in the absence or presence of Triton X-100 [1 mg·(mg of Chl)⁻¹]. After SDS/PAGE (10–16% acrylamide) and blotting on to PVDF membranes, NDH-F and its V8 derivatives were revealed by a monospecific NDH-F antibody. The approximate molecular mass of V8 peptides is indicated on the right.