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All analytes [1 (7.5 μM), 2 (3.75 μM) and 3 (1.675 μM)] of the 5E scFv, which are shown in the inset, were injected over immobilized hPDIR (A) or hPDI (B) on the sensor chip. Progress in the binding of the 5E scFv to immobilized hPDI and hPDIR was assessed by following the increase in the signal [expressed in terms of RU (resonance unit)].
