Table 2. Dissociation constants (KD) and isomerase activities of PDI family proteins and their mutants bearing one CGHCK sequence.
| Ligand | KD (M)* | Order of affinity | Isomerase activity (%) | Order of activity |
|---|---|---|---|---|
| hPDI | 4.06×10−7 | 100.00 | ||
| hPDI-m2 | 2.50×10−7 | 1 | 92.83 | 1 |
| hPDI-m1 | 2.36×10−4 | 9 | 53.46 | 5 |
| hPDI-m12 | N.D.† | 0.00 | ||
| hP5 | 3.66×10−4 | 45.00 | ||
| hP5-m2Q40K | 2.41×10−5 | 7 | 60.92 | 4 |
| hP5-m1 | 3.83×10−5 | 8 | 22.50 | 6 |
| hP5-m12 | N.D. | 0.00 | ||
| hPDIR | 2.36×10−6 | 2.50 | ||
| hPDIR-m13 | 4.09×10−6 | 5 | 1.38 | 8 |
| hPDIR-m12-GH | 1.80×10−6 | 3 | 1.84 | 7 |
| hPDIR-m23-GH | 8.19×10−6 | 6 | 1.00 | 9 |
| hPDIR-m123 | N.D. | 0.00 | ||
| yPDI | 3.30×10−7 | 200.00 | ||
| yPDI-m2 | 4.05×10−6 | 4 | 62.40 | 3 |
| yPDI-m1 | 7.80×10−7 | 2 | 86.88 | 2 |
| yPDI-m12 | N.D. | 0.00 |
* Determined by using the 5E scFv. Results are the means for two separate experiments.
† N.D., not detected.