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. 2004 Aug 10;382(Pt 1):191–198. doi: 10.1042/BJ20031946

Figure 2. Fraction of unfolded protein fu as a function of temperature for BthTx-I and substrate-binding region mutants.

Figure 2

Native BthTx-I (□), wild-type recombinant BthTx-I (♦) and the mutants L5F (▾), V102A (•), L106F (○), L5F/V102A (crossed diamonds), V102A/L106F (+) and L5F/V102A/L106F (▵). The inset presents the values of melting temperature Tm of BthTx-I before and after site-directed mutagenesis of residues in the substrate-binding region; n.d., value not determined.