Figure 4. Release of entrapped calcein from liposomes composed of EYPC/DMPA at a 9:1 molar ratio 150 s after mixing with native BthTx-I (Nat), wild-type recombinant (Rec) and substrate-binding-site mutants at a protein/lipid ratio of 1:400.
The final protein concentration in all experiments was 3 μg·ml−1, and the results are expressed as mean percentage of total calcein release after the addition of 5 mM Triton X-100.