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. 2004 Aug 10;382(Pt 1):261–268. doi: 10.1042/BJ20040285

Figure 4. FRK/RAK knockdown by siRNA.

Figure 4

(A) FRK/RAK protein expression after transfection of FRK/RAK-overexpressing RINm5F cells with siRNA against murine FRK/RAK. Scrambled (Scr) oligonucleotide control transfection is shown. The blot was also probed for c-Abl, c-SRC and p42-p44 ERK to assess loading. (B) Islet transfection efficiency. Freshly isolated islets were transfected with a fluorescently scrambled siRNA oligonucleotide. On the next day, the islets were dispersed and analysed by FACS. A population of cells with increased fluorescence is indicated by R2. R1 indicates the viable untransfected cell population. (C) Islet cell viability with or without cytokine addition (50 units/ml IL-1β +1000 units/ml IFN-γ) after transfection with murine FRK/RAK RNAi. Results are the means±S.E.M. for three independent experiments. *P<0.05 with a paired Student's t test.