Table 1. Insulin secretion, insulin content, NO production and DNA content of FRK/RAK +/+ and −/− islets in response to cytokine exposure for 18 h.
Values are means±S.E.M. for the number of observations given in parentheses.
| Insulin secretion | |||||
|---|---|---|---|---|---|
| 1.7 mM glucose (ng/10 islets) | 16.7 mM glucose (ng/10 islets) | Insulin content (ng/30 islets) | NO production [μmol·(25 islets)−1·(18 h)−1] | Islet DNA (ng/30 islets) | |
| FRK/RAK +/+ | 4.2±0.8 (6) | 39±8.0 (6) | 448±62 (6) | 0.4±0.1 (7) | 672±67 (4) |
| FRK/RAK +/+ 75 units/ml IL-1β+1500 units/ml IFN-γ | 8.3±2.2 (6) | 17±5.9 (6) | 360±60 (6) | 1.4±0.9 (7) | 552±96 (4) |
| FRK/RAK −/− | 3.8±0.9 (6) | 46±4.6 (6) | 663±155 (6) | 0.4±0.1 (7) | 610±148 (4) |
| FRK/RAK −/− 75 units/ml IL-1β+1500 units/ml IFN-γ | 4.8±1.0 (6) | 6.6±1.2 (6) | 419±94 (6) | 1.3±0.2 (7) | 568±132 (4) |