Figure 4. 1H-NMR spectra of CS-A and CS derivatives.
(a) Intact CS-A, (b) partially O-sulphated CS-A, (c) fully O-sulphated CS-A, (d) de-O-sulphated CS-A recorded in 2H2O at 333 K (or 60 °C). The letters in the spectra refer to the corresponding residues in the structures. Assignments were determined using 2D NMR [27].